Composite

Part:BBa_K1685011:Design

Designed by: ITB_Indonesia 2015   Group: iGEM15_ITB_INDONESIA   (2015-09-16)


Rhamnolipid production


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 225
    Illegal BamHI site found at 785
    Illegal XhoI site found at 961
    Illegal XhoI site found at 2247
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1140
    Illegal NgoMIV site found at 1861
    Illegal NgoMIV site found at 1974
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 450
    Illegal BsaI site found at 1490
    Illegal BsaI.rc site found at 634


Design Notes

We include the scar sequences between RBS-coding and coding-terminator in hope that it will function properly.


Source

The part was chemically synthesised. The sequences were based on parts available in Registry.

References